OriDB Curated Paper

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Systematic identification of fragile sites via genome-wide location analysis of gamma-H2AX.

Rachel K Szilard, Pierre-Etienne Jacques, Louise Laramée, Benjamin Cheng, Sarah Galicia, Alain R Bataille, ManTek Yeung, Megan Mendez, Maxime Bergeron, François Robert, Daniel Durocher

Nat. Struct. Mol. Biol. (2010), 17(3):299-305Data to DownloadPubMed | PubMed Central | Nat. Struct. Mol. Biol.

Phosphorylation of histone H2AX is an early response to DNA damage in eukaryotes. In Saccharomyces cerevisiae, DNA damage or replication-fork stalling results in phosphorylation of histone H2A yielding gamma-H2A (yeast gamma-H2AX) in a Mec1 (ATR)- and Tel1 (ATM)-dependent manner. Here, we describe the genome-wide location analysis of gamma-H2A as a strategy to identify loci prone to engaging the Mec1 and Tel1 pathways. Notably, gamma-H2A enrichment overlaps with loci prone to replication-fork stalling and is caused by the action of Mec1 and Tel1, indicating that these loci are prone to breakage. Moreover, about half the sites enriched for gamma-H2A map to repressed protein-coding genes, and histone deacetylases are necessary for formation of gamma-H2A at these loci. Finally, our work indicates that high-resolution mapping of gamma-H2AX is a fruitful route to map fragile sites in eukaryotic genomes.

OriDB annotation of this paper:

ARS assay

None curated.

2D gel

None curated.

ChIP of replication origin proteins

ARS102.5, ARS103, ARS at I-10, ARS at I-17, ARS104, ARS105, ARS106, ARS107, ARS108, ARS109, ARS110, ARS at I-198, ARS112, ARS at I-230, ARS201.5, ARS202, ARS203, ARS206, ARS207, ARS207.1, ARS at II-188, ARS207.5, ARS208, ARS209, ARS211, ARS212, ARS213, ARS214, ARS215, ARS216, ARS217, ARS218, ARS219, ARS220, ARS at II-627, ARS221, ARS221.5, ARS222, ARS223, ARS224, ARS225, ARS227, ARS228, ARS228.5, ARS229, ARS301, ARS302, ARS302, ARS302, ARS303, ARS320, ARS302, ARS305, ARS306, ARS307, ARS308, ARS309, ARS at III-163, ARS310, ARS313, ARS314, ARS at III-200, ARS at III-224, ARS315, ARS316, ARS317, ARS318, ARS at IV-9, ARS404, ARS at IV-51, ARS405, ARS at IV-96, ARS406, ARS407, ARS408, ARS409, ARS at IV-220, ARS409.3, ARS409.5, ARS410, ARS412, ARS413, ARS414, ARS415, ARS416, ARS at IV-477, ARS417, ARS417.5, ARS418, ARS419, ARS420, ARS421, ARS at IV-689, ARS422, ARS at IV-721, ARS422.5, ARS423, ARS425, ARS at IV-899, ARS at IV-899, ARS428, ARS429, ARS430, ARS at IV-1022, ARS430.5, ARS431, ARS431.5, ARS432, ARS432.5, ARS433, ARS at IV-1275, ARS434, ARS435, ARS437, ARS at IV-1380, ARS439.5, ARS440, ARS at IV-1429, ARS441, ARS446, ARS447, ARS at IV-1524, ARS503, ARS504.2, ARS at V-18, ARS507, ARS508, ARS at V-110, ARS510, ARS511, ARS511.5, ARS512, ARS at V-256, ARS513.5, ARS513.7, ARS514, ARS at V-317, ARS516, ARS517, ARS at V-418, ARS518, ARS520, ARS521, ARS522, ARS523, ARS523, ARS600.3, ARS600.4, ARS600.4, ARS601, ARS602, ARS603, ARS603.5, ARS604, ARS605, ARS606, ARS607, ARS608, ARS609, ARS at VI-269, ARS610, ARS700.5, ARS at VII-33, ARS702, ARS704, ARS at VII-115, ARS706, ARS707, ARS709, ARS710, ARS711, ARS712, ARS714, ARS at VII-319, ARS716, ARS717, ARS718, ARS719, ARS720, ARS at VII-553, ARS721, ARS722, ARS at VII-576, ARS726, ARS727, ARS728, ARS729, ARS731, ARS731.5, ARS732, ARS733, ARS734, ARS735.5, ARS at VII-1074, ARS131a, ARS131n, ARS802, ARS804, ARS805, ARS805.7, ARS807, ARS809, ARS810, ARS at VIII-215, ARS813, ARS815, ARS at VIII-345, ARS816, ARS at VIII-381, ARS818, ARS818.5, ARS820, ARS821, ARS822, ARS823, ARS at VIII-551, ARS at VIII-553, ARS824, ARS at IX-8, ARS at IX-17, ARS at IX-31, ARS905, ARS907, ARS908, ARS909, ARS911, ARS911.5, ARS912, ARS at IX-190, ARS913, ARS913.5, ARS914, ARS at IX-249, ARS916, ARS919, ARS920, ARS922, ARS at IX-439, ARS1002, ARS1003, ARS1004, ARS1005, ARS1006, ARS1007, ARS1007.5, ARS1009, ARS1009.5, ARS1010, ARS1011, ARS1012, ARS1013, ARS at X-406, ARS1014, ARS1015, ARS1017, ARS1018, ARS1019, ARS at X-644, ARS1020, ARS1021, ARS1022, ARS1024, ARS at XI-16, ARS at XI-24, ARS1102.5, ARS1103, ARS1104.5, ARS1106, ARS1106.3, ARS1106.7, ARS at XI-236, ARS1107, ARS1109, ARS1112, ARS1113, ARS1114, ARS1114.5, ARS at XI-463, ARS at XI-501, ARS1116, ARS1117, ARS at XI-561, ARS1118, ARS1120, ARS at XI-633, ARS1123, ARS at XI-662, ARS at XII-12, ARS at XII-31, ARS1203, ARS1205, ARS1206, ARS1206.5, ARS at XII-140, ARS1209, ARS1210, ARS1211, ARS1212, ARS1212.5, ARS1213, ARS1215, ARS at XII-419, ARS1216, ARS1216.5, ARS at XII-461, ARS at XII-468, ARS1217, ARS at XII-599, ARS1218, ARS1219, ARS at XII-618, ARS1219.7, ARS1220, ARS1222, ARS1223, ARS at XII-786, ARS1226, ARS1227, ARS1227.5, ARS1229, ARS1231, ARS1232, ARS1233, ARS1234, ARS1235, ARS at XII-1056, ARS1303, ARS1304, ARS1305, ARS1307, ARS1307.5, ARS at XIII-227, ARS1309, ARS at XIII-278, ARS1310, ARS at XIII-342, ARS1312, ARS at XIII-417, ARS1315, ARS1316, ARS1319, ARS1320, ARS1322, ARS1323, ARS1324, ARS1325, ARS1326, ARS1327, ARS1328, ARS1329, ARS1330, ARS1331.7, ARS1332, ARS1405, ARS at XIV-36, ARS1406, ARS at XIV-85, ARS1407, ARS1410, ARS at XIV-137, ARS1411, ARS1412, ARS1413, ARS1414, ARS1415, ARS1311.7, ARS at XIV-358, ARS at XIV-359, ARS1417, ARS1419, ARS1420, ARS1421, ARS1424, ARS1426, ARS1427, ARS1428, ARS at XIV-731, ARS at XIV-734, ARS1428.5, ARS1429, ARS at XV-11, ARS at XV-19, ARS1506.5, ARS1507, ARS1508, ARS1509.3, ARS1510, ARS1510.5, ARS at XV-269, ARS1511, ARS1512, ARS1513, ARS at XV-348, ARS at XV-354, ARS1513.5, ARS at XV-464, ARS1514, ARS1515.5, ARS1516, ARS1517, ARS at XV-605, ARS at XV-618, ARS1519, ARS at XV-670, ARS1520, ARS1521, ARS1523, ARS1524, ARS1525, ARS1526, ARS at XV-900, ARS1528, ARS1529, ARS at XV-999, ARS1529.5, ARS at XV-1081, ARS1601, ARS1604, ARS1605, ARS at XVI-91, ARS1607, ARS at XVI-142, ARS at XVI-163, ARS at XVI-191, ARS1609, ARS at XVI-251, ARS at XVI-262, ARS1614, ARS at XVI-299, ARS at XVI-318, ARS1617, ARS1618, ARS1619, ARS at XVI-428, ARS1620.5, ARS at XVI-500, ARS1621, ARS at XVI-553, ARS1622, ARS1622.7, ARS1623, ARS1624, ARS1625, ARS1626, ARS1626.5, ARS1627, ARS1628, ARS at XVI-864, ARS1630, ARS at XVI-930, ARS1631, ARS at XVI-942

Replication timing

None curated.

Replication in hydroxyurea

None curated.

Predicted origins

None curated.

Confirmed sequence element

None curated.

Predicted sequence element

None curated.

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