OriDB - ARS at XII-140

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Status: Dubious ARS: Identified by 10 genome-wide studies.

Genomic Location: Chr12:139293-140447
Within gene: YLL005C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -151.3 ΔG° (kcal/mol) at location 139303.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 21.8 min.
Yabuki et al. (2002) — Trep: 22.9 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XII-140 has unique ID: 400

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ARS at XII-140 has unique ID: 400

Studies that cloned this origin

None curated.

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr12:138184-139163

Xu et al. (2006): Chr12:139295-140445

Shor et al. (2009): Chr12:139300-140300 (orc2-1/wt peak ratio: 0.60)

Szilard et al. (2010): Chr12:139795-139805

MÃ¼ller et al. (2010): Chr12:139475-140075 (orc1-bah-delta/wt peak ratio: 0.42)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr12:139812 (Trep: 21.8 min.) (Confidence: 7)

Yabuki et al. (2002): Chr12:139304 (Trep: 22.9 min.)

Alvino et al. (2007): Chr12:140000 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr12:139750 (Activity detected in: wild-type, rad53)

CrabbÃ© et al. (2010): Chr12:139293-140447 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)