ARS1007

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 9 genome-wide studies.

Genomic Location: Chr10:113226-113828
Within tandem intergenic space between YJL163C and YJL162C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -153.6 ΔG° (kcal/mol) at location 113246.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 32.8 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-114 has unique ID: 333

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ARS at X-114 has unique ID: 333

Studies that cloned this origin

Wyrick et al. (2001): Chr10:113226-113828

Studies that analyzed this origin by 2D gel

Aparicio et al. (2004): Chr10:111113-116517

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:113327-114175

Xu et al. (2006): Chr10:111895-116320

Shor et al. (2009): Chr10:113100-114700 (orc2-1/wt peak ratio: 0.51)

Szilard et al. (2010): Chr10:113765-113775

Müller et al. (2010): Chr10:112925-115625 (orc1-bah-delta/wt peak ratio: 0.76)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:108252 (Trep: 32.8 min.) (Confidence: 9)

Alvino et al. (2007): Chr10:113250 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:114000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr10:113226-113828 (Activity detected in: mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at X-114 has unique ID: 333

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

T

T

A

A

A

T

A

T

A

T

A

T

T

T

A

G

T

T

A

T

G

G

A

A

A

T

T

C

A

A

T

A

A

A

Xu et al. (2006):

T

T

T

T

T

T

A

C

T

T

T

T

A

C

C

A

T

T

T

T

C

T

G

T

A

A

G

A

A

T

T

T

T

C

Eaton et al. (2010):

T

A

A

A

T

A

T

A

T

A

T

T

T

A

G

T

T

A

T

G

G

A

A

A

T

T

C

A

A

T

A

A

A

ACS LOGO:

ARS at X-114 has unique ID: 333

Alignments from the UCSC genome browser

ARS at X-114 has unique ID: 333

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-114 has unique ID: 333

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Wyrick et al. (2001): PubMed | Science

Studies that analyzed this origin by 2D gel

Aparicio et al. (2004): PubMed | PubMed Central | Mol. Cell. Biol.

Studies that confirmed an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at X-114 has unique ID: 333