ARS815

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr8:296882-297475
Within tandem intergenic space between YHR096C and YHR097C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -162.4 ΔG° (kcal/mol) at location 296922.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 25.8 min.
Yabuki et al. (2002) — Trep: 22.4 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VIII-297 has unique ID: 279

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ARS at VIII-297 has unique ID: 279

Studies that cloned this origin

Shor et al. (2009): Chr8:296882-297475

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr8:296449-297387

Xu et al. (2006): Chr8:296235-298220

Shor et al. (2009): Chr8:296700-298100 (orc2-1/wt peak ratio: 0.81)

Szilard et al. (2010): Chr8:297205-297215

Müller et al. (2010): Chr8:296750-298400 (orc1-bah-delta/wt peak ratio: 0.65)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr8:297501 (Trep: 25.8 min.) (Confidence: 9)

Yabuki et al. (2002): Chr8:298585 (Trep: 22.4 min.)

Alvino et al. (2007): Chr8:296440 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr8:297250 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr8:296233-298222 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VIII-297 has unique ID: 279

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Eaton et al. (2010):

T

T

T

C

T

T

T

A

C

T

T

T

T

T

G

C

T

A

G

C

A

G

A

T

G

A

T

T

A

C

T

T

T

ACS LOGO:

ARS at VIII-297 has unique ID: 279

Alignments from the UCSC genome browser

ARS at VIII-297 has unique ID: 279

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VIII-297 has unique ID: 279

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VIII-297 has unique ID: 279