ARS104

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr1:30946-31184
Within divergent intergenic space between YAL063C and YAL062W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -146.8 ΔG° (kcal/mol) at location 31176.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 28.3 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at I-31 has unique ID: 3

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ARS at I-31 has unique ID: 3

Studies that cloned this origin

Nieduszynski et al. (2006): Chr1:30946-31184

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr1:30371-31573

Xu et al. (2006): Chr1:30133-31597

Shor et al. (2009): Chr1:30400-31400 (orc2-1/wt peak ratio: 0.48)

Szilard et al. (2010): Chr1:30875-30885

Müller et al. (2010): Chr1:30425-31550 (orc1-bah-delta/wt peak ratio: 0.59)

Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr1:31269 (Trep: 28.3 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr1:31000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr1:30946-31184 (Activity detected in: ddc1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at I-31 has unique ID: 3

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

T

A

T

T

T

T

T

A

A

G

T

T

T

T

G

T

Xu et al. (2006):

T

A

T

T

T

T

T

A

A

G

T

T

T

T

G

T

T

G

G

G

T

C

T

C

T

G

A

G

A

A

C

T

T

T

Eaton et al. (2010):

T

A

T

T

T

T

T

A

A

G

T

T

T

T

G

T

T

G

G

G

T

C

T

C

T

G

A

G

A

A

C

T

T

ACS LOGO:

ARS at I-31 has unique ID: 3

Alignments from the UCSC genome browser

ARS at I-31 has unique ID: 3

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at I-31 has unique ID: 3

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at I-31 has unique ID: 3