Confirmed ARS at VII-978

ARS733

Other names: proARS733

Status: Confirmed ARS: Confirmed by ARS assay and identified by 11 genome-wide studies.

Genomic Location: Chr7:977730-977979
Within convergent intergenic space between YGR243W and YGR244C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -143.5 ΔG° (kcal/mol) at location 977730.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 34.0 min.
Yabuki et al. (2002) — Trep: 27.0 min.
Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VII-978 has unique ID: 255

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ARS at VII-978 has unique ID: 255

Studies that cloned this origin

Nieduszynski et al. (2006): Chr7:977730-977979


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr7:977771-978031

Xu et al. (2006): Chr7:977205-978745

Shor et al. (2009): Chr7:977400-978300 (orc2-1/wt peak ratio: 0.36)

Szilard et al. (2010): Chr7:977865-977875

Müller et al. (2010): Chr7:977600-978200 (orc1-bah-delta/wt peak ratio: 0.29)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr7:982673

Raghuraman et al. (2001): Chr7:971168 (Trep: 34.0 min.) (Confidence: 2)

Yabuki et al. (2002): Chr7:979647 (Trep: 27.0 min.)

Alvino et al. (2007): Chr7:978330 (Peak first observed at 15.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr7:978333 (Activity detected in: rad53)

Crabbé et al. (2010): Chr7:977730-977979 (Activity detected in: ddc1, rad24, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at VII-978 has unique ID: 255

Studies that confirmed an essential ACS element

  Nieduszynski et al. (2006):       TTTTTTATATTTTTCC                   

Studies that predicted an essential ACS element

  Xu et al. (2006):      AATTTTTTTATATTTTTCCATCTTTTGATGTAAT  
ACS LOGO:   ACS_logo  

ARS at VII-978 has unique ID: 255

Alignments from the UCSC genome browser

Confirmed ACS
S. cerevisiaeTTCTTTTTTTTTTTTTTAATTTTTTTATATTTTTCCATCTTTTGATGTAATATAGA
S. kudriavzeviiTTC---TTATTCTTGAT--TTTCTTTATAATTTT--ACCTTTTGTTGTAATTTTAA
S. mikataeTTC---TTAATCTCGATGATTTTTACATTTCTTCACAT-TTTTGATGTAATATAGA
S. paradoxusTTC---TTATTCTTGAT--TTTTTTTATATTTTTTCACCTTTTGATGTAATATAAA
S. bayanusTTC---TTATTCTTTTC--TGTTTTTACTTTTTTTTACATTTAGATGTAAAATATG
***:::**::*:*:::*:*:*::*:::**::****:*:*****::*::

ARS at VII-978 has unique ID: 255

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VII-978 has unique ID: 255

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at VII-978 has unique ID: 255