ARS602

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 10 genome-wide studies.

Genomic Location: Chr6:32666-33247
Within tandem intergenic space between YFL051C and YFL050C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -154.0 ΔG° (kcal/mol) at location 33246.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 42.4 min.
Yabuki et al. (2002) — Trep: 32.1 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VI-33 has unique ID: 650

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ARS at VI-33 has unique ID: 650

Studies that cloned this origin

Shirahige et al. (1993): Chr6:32666-33247

Shirahige et al. (1993): Chr6:32472-32995

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): Chr6:29727-34843

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr6:31906-33272

Xu et al. (2006): Chr6:31647-34439

Shor et al. (2009): Chr6:31500-33800 (orc2-1/wt peak ratio: 0.58)

Szilard et al. (2010): Chr6:32875-32885

Müller et al. (2010): Chr6:31475-33800 (orc1-bah-delta/wt peak ratio: 0.51)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr6:37304 (Trep: 42.4 min.) (Confidence: 9)

Yabuki et al. (2002): Chr6:35863 (Trep: 32.1 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr6:34000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr6:32666-33247

Crabbé et al. (2010): Chr6:32472-32995 (Activity detected in: mrc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VI-33 has unique ID: 650

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

C

T

A

A

T

T

T

C

C

A

T

T

T

T

T

G

A

G

T

T

T

A

G

G

C

G

G

T

G

C

T

T

T

C

Xu et al. (2006):

A

A

T

T

T

A

T

A

C

G

T

T

T

A

G

T

T

T

T

A

A

C

A

T

C

A

T

C

A

C

A

A

T

G

Eaton et al. (2010):

A

A

T

T

T

A

T

A

C

G

T

T

T

A

G

T

T

T

T

A

A

C

A

T

C

A

T

C

A

C

A

A

T

ACS LOGO:

ARS at VI-33 has unique ID: 650

Alignments from the UCSC genome browser

ARS at VI-33 has unique ID: 650

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VI-33 has unique ID: 650

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Shirahige et al. (1993): PubMed | PubMed Central

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): PubMed

Studies that confirmed an essential ACS element

Shirahige et al. (1993): PubMed | PubMed Central

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VI-33 has unique ID: 650