ARS604

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 7 genome-wide studies.

Genomic Location: Chr6:127745-128066
Within gene: YFL007W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -145.8 ΔG° (kcal/mol) at location 127865.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 21.5 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VI-128 has unique ID: 205

DetailedDefaultZoomed Out

Loading - Please wait...

ARS at VI-128 has unique ID: 205

Studies that cloned this origin

Shirahige et al. (1993): Chr6:127745-128066

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): Chr6:125914-132085

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr6:127725-129315

Shor et al. (2009): Chr6:127400-128600 (orc2-1/wt peak ratio: 0.07)

Szilard et al. (2010): Chr6:128235-128245

Müller et al. (2010): Chr6:127475-128375 (orc1-bah-delta/wt peak ratio: 1.34)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr6:122427 (Trep: 21.5 min.) (Confidence: 9)

Alvino et al. (2007): Chr6:122670 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr6:127745-128066 (Activity detected in: rev3 rad30, eco1, ctf4, ddc1, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VI-128 has unique ID: 205

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

T

C

A

T

T

T

T

A

C

G

T

T

T

T

G

T

T

A

C

A

C

A

A

A

T

C

C

A

A

T

C

G

A

A

Eaton et al. (2010):

T

C

A

T

T

T

T

A

C

G

T

T

T

T

G

T

T

A

C

A

C

A

A

A

T

C

C

A

A

T

C

G

A

ACS LOGO:

ARS at VI-128 has unique ID: 205

Alignments from the UCSC genome browser

ARS at VI-128 has unique ID: 205

These notes are manually curated. To submit notes for this replication origin site please contact us.

Thursday, 17th August 2006 - Conrad Nieduszynski:

2D gel studies indicate that ARS604 has barely detectable origin activity, while its two flanking origins (ARS603.5 and ARS605) have robust origin activity (see Yamashita et al., 1997 and Friedman et al., 1997 in the References tab). [Thanks to Joel Huberman for this information.]

ARS at VI-128 has unique ID: 205

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Shirahige et al. (1993): PubMed | PubMed Central

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): PubMed

Studies that confirmed an essential ACS element

Shirahige et al. (1993): PubMed | PubMed Central

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VI-128 has unique ID: 205