Confirmed ARS at X-654

ARS1020

Other names: proARS1020

Status: Confirmed ARS: Confirmed by ARS assay and identified by 7 genome-wide studies.

Genomic Location: Chr10:654069-654309
Within tandem intergenic space between YJR124C and YJR125C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -143.0 ΔG° (kcal/mol) at location 654069.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 24.4 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-654 has unique ID: 353

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ARS at X-654 has unique ID: 353

Studies that cloned this origin

Nieduszynski et al. (2006): Chr10:654069-654309


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:653927-654426

Xu et al. (2006): Chr10:653440-655430

Szilard et al. (2010): Chr10:654315-654325


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:655265 (Trep: 24.4 min.) (Confidence: 8)

Alvino et al. (2007): Chr10:653000 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:654000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr10:654069-654309 (Activity detected in: rev3 rad30, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at X-654 has unique ID: 353

Studies that confirmed an essential ACS element

  Xu et al. (2006):         ATTTACATTTT                      

Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTATTTACATTTTGGT                   
ACS LOGO:   ACS_logo  

ARS at X-654 has unique ID: 353

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeAAGAAGGGCCCTTTTCAAATTTATTTACATTTTGGTCATTTGAAAATACTTTACTT
S. kudriavzeviiAAGGAAAATCTTTCACAAGATTATTTACATCTTGGTCAATTGCAAATATTTTATAC
S. mikataeGAAAGAAACGCATCCAAAGATTATTTACATTTTGGTCATTTACAAACATTTTAATT
S. paradoxusTAGAAGGGCGCATCTCCAGTTTATTTACATTTTGGTCATTCAAAACCATTTTAATT
S. bayanusTGAAAATGTTCACCATAAAAATATTAACATTTTGGTCGATTACTAATACTTTA-TG
:::::::*:****:****:******:*::*:*****::

ARS at X-654 has unique ID: 353

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-654 has unique ID: 353

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


ARS at X-654 has unique ID: 353