OriDB - ARS513.5

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Origin

Status: Confirmed ARS: Confirmed by ARS assay and identified by 6 genome-wide studies.

Genomic Location: Chr5:276127-276526
Within tandem intergenic space between YER060W and YER060W-A.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -145.7 ΔG° (kcal/mol) at location 276257.

Time of Origin Replication (Trep): This site was not identified as an origin by any curated timing study (Raghuraman et al. (2001); Yabuki et al. (2002); Alvino et al. (2007)), suggesting that this site is not chromosomally active for replication initiation.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at V-276 has unique ID: 180

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ARS at V-276 has unique ID: 180

Studies that cloned this origin

Shor et al. (2009): Chr5:276127-276526

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr5:275575-278875

Shor et al. (2009): Chr5:275700-276600 (orc2-1/wt peak ratio: 0.37)

Szilard et al. (2010): Chr5:276125-276135

Müller et al. (2010): Chr5:275825-276650 (orc1-bah-delta/wt peak ratio: 0.95)

Studies that measured the replication time of this origin

The replication time of origin has not be reported by any curated study.

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr5:276666 (Activity detected in: rad53)

Crabbé et al. (2010): Chr5:276808-277308 (Activity detected in: elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)