Confirmed ARS at X-684

ARS1021

Other names: ARS121, proARS1021

Status: Confirmed ARS: Confirmed by ARS assay and identified by 11 genome-wide studies.

Genomic Location: Chr10:683328-683817
Within divergent intergenic space between YJR137C and YJR138W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -144.0 ΔG° (kcal/mol) at location 683598.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 16.1 min.
Yabuki et al. (2002) — Trep: 18.3 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-684 has unique ID: 354

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ARS at X-684 has unique ID: 354

Studies that cloned this origin

Nieduszynski et al. (2006): Chr10:683328-683817


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:682974-684256

Xu et al. (2006): Chr10:683165-684845

Shor et al. (2009): Chr10:683300-684600 (orc2-1/wt peak ratio: 1.00)

Szilard et al. (2010): Chr10:683785-683795

Müller et al. (2010): Chr10:683225-684875 (orc1-bah-delta/wt peak ratio: 0.70)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:684266 (Trep: 16.1 min.) (Confidence: 9)

Yabuki et al. (2002): Chr10:682933 (Trep: 18.3 min.)

Alvino et al. (2007): Chr10:684220 (Peak first observed at 10.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:681000 (Activity detected in: rad53)

Feng et al. (2006): Chr10:685333

Crabbé et al. (2010): Chr10:683328-683817 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at X-684 has unique ID: 354

Studies that confirmed an essential ACS element

  Nieduszynski et al. (2006):        TTGTTTAACATTAGT                   

Studies that predicted an essential ACS element

  Xu et al. (2006):      TTTTGTGAGAAAATATATTTTGGATGAATAGCAG  
  Eaton et al. (2010):      TTTGTTTAACATTAGTTTCAAATTAACAGCTTT   
ACS LOGO:   ACS_logo  

ARS at X-684 has unique ID: 354

Alignments from the UCSC genome browser

Confirmed ACS
S. cerevisiaeTCAATTTGAAGTGAGATGTTTTGTTTAACATTAGTTTCAAATTAACAGCTTTTGT
S. kudriavzeviiTTAAGGGGAAACAGTATGAGGTATTTAATATTTTTTTCCCAATTTTATCTTTTCT
S. mikataeCCAAGATTGAGCAGAATGCTTTATTTAATATTTGTTTCCAAATGACAGCTTTCAT
S. paradoxusTTATAGTGAAGCAAGATATTTTATTTAATATTAGTTTTCAATTGATAGTTTTT-T
S. bayanusTTAGAACGAA---AGATTATTTATTTAATATTTGTTTCCAGTTCTTAGCTTTT-T
:*::::*::**::*:*****:***:***::::*:*::***::*

ARS at X-684 has unique ID: 354

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-684 has unique ID: 354

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at X-684 has unique ID: 354