Confirmed ARS at X-299

ARS1010

Other names: proARS1010

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr10:298471-298952
Within convergent intergenic space between YJL076W and YJL074C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -145.4 ΔG° (kcal/mol) at location 298911.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 33.2 min.
Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-299 has unique ID: 340

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ARS at X-299 has unique ID: 340

Studies that cloned this origin

Wyrick et al. (2001): Chr10:298471-298952


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:298572-298853

Xu et al. (2006): Chr10:298095-299900

Szilard et al. (2010): Chr10:298775-298785

Müller et al. (2010): Chr10:298700-299300 (orc1-bah-delta/wt peak ratio: 0.98)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:304757 (Trep: 33.2 min.) (Confidence: 9)

Alvino et al. (2007): Chr10:305890 (Peak first observed at 15.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:300000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr10:298471-298952 (Activity detected in: mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at X-299 has unique ID: 340

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       CTTATTTACCTCTTTT                   
ACS LOGO:   ACS_logo  

ARS at X-299 has unique ID: 340

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeAATCTTCATGTAAACCAATTCTTATTTACCTCTTTTCCCAGCAAGCGGGCAACTTC
S. kudriavzeviiATTTTTCATGTAAACTAAT--TTATTCATCTCTACGATAATCA--TGATCAA-TTT
S. mikataeAATCTTCTTATA--CTAATTTTATCTAATTTCTTTACCAAGTTAGTGACTAAACTC
S. paradoxusAATCTTCATGTAAACTAGTTCTTACTCATCTCGTTCTCCATTGAGTAGTCAATCTC
S. bayanusATTTCTCATGTAAACTAATTCT-ATTTATCTCCTTCAATATTAAATAAGTAGATTT
**:**:*:**::*:*:*:*:**::**:::*::*::*

ARS at X-299 has unique ID: 340

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-299 has unique ID: 340

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Wyrick et al. (2001): PubMed | Science


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


ARS at X-299 has unique ID: 340