Confirmed ARS at XII-822

ARS1227

Other names: proARS1227

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr12:821907-822336
Within convergent intergenic space between YLR345W and YLR346C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -145.2 ΔG° (kcal/mol) at location 822317.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 32.4 min.
Yabuki et al. (2002) — Trep: 28.4 min.
Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XII-822 has unique ID: 433

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ARS at XII-822 has unique ID: 433

Studies that cloned this origin

Müller & Nieduszynski (2012): Chr12:821907-822336


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr12:822039-822286

Xu et al. (2006): Chr12:821405-823000

Shor et al. (2009): Chr12:821700-822500 (orc2-1/wt peak ratio: 0.50)

Szilard et al. (2010): Chr12:822205-822215

Müller et al. (2010): Chr12:821600-822725 (orc1-bah-delta/wt peak ratio: 0.51)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr12:816112 (Trep: 32.4 min.) (Confidence: 4)

Yabuki et al. (2002): Chr12:820804 (Trep: 28.4 min.)

Alvino et al. (2007): Chr12:817440 (Peak first observed at 15.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr12:821666 (Activity detected in: rad53)

Crabbé et al. (2010): Chr12:822201-822701 (Activity detected in: ctf8, ctf18, mrc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XII-822 has unique ID: 433

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Eaton et al. (2010):      TATTTTTATGTTTAAGAAAGGATTAAGAACAAT   
ACS LOGO:   ACS_logo  

ARS at XII-822 has unique ID: 433

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at XII-822 has unique ID: 433

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XII-822 has unique ID: 433

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Müller & Nieduszynski (2012): PubMed | Genome Res.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at XII-822 has unique ID: 433