No systematic name assigned

Status: Dubious ARS: Identified by 8 genome-wide studies.

Genomic Location: Chr3:159363-166363
Resolution insufficient to assign intergenic space.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -171.2 ΔG° (kcal/mol) at location 163673.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 24.1 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-163 has unique ID: 77

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ARS at III-163 has unique ID: 77

Studies that cloned this origin

None curated.

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:166050-167084

Xu et al. (2006): Chr3:165855-167585

Szilard et al. (2010): Chr3:166745-166755

Müller et al. (2010): Chr3:166475-167075 (orc1-bah-delta/wt peak ratio: 0.64)

Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr3:162863 (Trep: 24.1 min.)

Alvino et al. (2007): Chr3:163220 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:166666 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr3:166494-167340 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at III-163 has unique ID: 77

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

None curated.

ACS LOGO:

ARS at III-163 has unique ID: 77

Alignments from the UCSC genome browser

ARS at III-163 has unique ID: 77

These notes are manually curated. To submit notes for this replication origin site please contact us.

Friday, 18th August 2006 - Conrad Nieduszynski:

Status manually changed to 'Dubious'. Carol Newlon's lab report that there is no detectable ARS activity in this region. [Thanks to Carol Newlon for this information.]

ARS at III-163 has unique ID: 77

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

None identified.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

None identified.

ARS at III-163 has unique ID: 77