Confirmed ARS at XVI-777

ARS1626.5

Other names: ARS1635

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr16:776921-777152
Within divergent intergenic space between TC(GCA)P2 and YPR121W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -145.0 ΔG° (kcal/mol) at location 776921.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 15.9 min.
Yabuki et al. (2002) — Trep: 22.0 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XVI-777 has unique ID: 630

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ARS at XVI-777 has unique ID: 630

Studies that cloned this origin

Nieduszynski et al. (2006): Chr16:776921-777152


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr16:775385-779275

Shor et al. (2009): Chr16:776500-777800 (orc2-1/wt peak ratio: 0.57)

Szilard et al. (2010): Chr16:777005-777015

Müller et al. (2010): Chr16:776150-777875 (orc1-bah-delta/wt peak ratio: 0.64)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr16:772637 (Trep: 15.9 min.) (Confidence: 9)

Yabuki et al. (2002): Chr16:777383 (Trep: 22.0 min.)

Alvino et al. (2007): Chr16:778110 (Peak first observed at 10.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr16:778750 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr16:776921-777152 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XVI-777 has unique ID: 630

Studies that confirmed an essential ACS element

  Nieduszynski et al. (2006):        TTATTTATATTTTGG                   

Studies that predicted an essential ACS element

  Xu et al. (2006):      TATTTATTTATATTTTGGCATTACTCTTCATCAT  
  Eaton et al. (2010):      TTTATTTATATTTTGGCATTACTCTTCATCATT   
ACS LOGO:   ACS_logo  

ARS at XVI-777 has unique ID: 630

Alignments from the UCSC genome browser

Confirmed ACS
S. cerevisiaeTGATTTTCATATTTATTTATTTATTTATATTTTGGCATTACTCTTCATCATTTTT
S. kudriavzeviiAAAATTATATATTCG-TTATTTATTAGGGCCAATTTG-------CTAC----TTT
S. mikataeTGATTTCCATATCCATTTATTTATTTGTATTTTAATG-------TCGCCATTTTC
S. paradoxusTGTTTTTTATATTTAATTATTTATTTGGGCTTAATTG-------TTGCCGTTCTT
::::******::*********::::::::*:

ARS at XVI-777 has unique ID: 630

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XVI-777 has unique ID: 630

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at XVI-777 has unique ID: 630