Confirmed ARS at II-774

ARS227

Other names: proARS227

Status: Confirmed ARS: Confirmed by ARS assay and identified by 7 genome-wide studies.

Genomic Location: Chr2:773918-774348
Within tandem intergenic space between YBR285W and YBR286W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -152.9 ΔG° (kcal/mol) at location 774128.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 32.7 min.
Alvino et al. (2007) — Peak first observed at 17.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at II-774 has unique ID: 60

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ARS at II-774 has unique ID: 60

Studies that cloned this origin

Xu et al. (2006): Chr2:773918-774348


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr2:773588-773878

Xu et al. (2006): Chr2:773025-774665

Szilard et al. (2010): Chr2:774035-774045


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr2:774563 (Trep: 32.7 min.) (Confidence: 9)

Alvino et al. (2007): Chr2:774400 (Peak first observed at 17.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr2:774000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr2:773918-774348 (Activity detected in: tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at II-774 has unique ID: 60

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Xu et al. (2006):      TGAAATGTCCGCCTCCATGCTATCGTCAGTGGGG  
ACS LOGO:   ACS_logo  

ARS at II-774 has unique ID: 60

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at II-774 has unique ID: 60

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at II-774 has unique ID: 60

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at II-774 has unique ID: 60