Confirmed ARS at VII-112

ARS704

Other names: proARS704

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr7:112080-112319
Within tandem intergenic space between TH(GUG)G1 and YGL203C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -138.0 ΔG° (kcal/mol) at location 112210.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 31.2 min.
Yabuki et al. (2002) — Trep: 28.1 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VII-112 has unique ID: 219

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ARS at VII-112 has unique ID: 219

Studies that cloned this origin

Nieduszynski et al. (2006): Chr7:112080-112319


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr7:112007-112477

Xu et al. (2006): Chr7:111735-115825

Shor et al. (2009): Chr7:111600-112900 (orc2-1/wt peak ratio: 0.56)

Szilard et al. (2010): Chr7:112195-112205

Müller et al. (2010): Chr7:111500-113300 (orc1-bah-delta/wt peak ratio: 0.44)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr7:112798 (Trep: 31.2 min.) (Confidence: 9)

Yabuki et al. (2002): Chr7:113647 (Trep: 28.1 min.)

Alvino et al. (2007): Chr7:111110 (Peak first observed at 10.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr7:112333 (Activity detected in: rad53)

Crabbé et al. (2010): Chr7:112080-112319 (Activity detected in: ddc1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at VII-112 has unique ID: 219

Studies that confirmed an essential ACS element

  Nieduszynski et al. (2006):       TTTATACGTTTATGTC                   

Studies that predicted an essential ACS element

  Xu et al. (2006):      ATATTTTTTCAGTTTTCGGACCATTTTTTGACAT  
  Xu et al. (2006):      TTTATACGTTTATGTCTATTTCAATGCAATTACG  
  Eaton et al. (2010):      ATTTTATACGTTTATGTCTATTTCAATGCAATT   
ACS LOGO:   ACS_logo  

ARS at VII-112 has unique ID: 219

Alignments from the UCSC genome browser

Confirmed ACS
S. cerevisiaeTCTAATTACTTGTATATTATTTTATACGTTTATGTCTATTTCAATGCAATTACGTG
S. mikataeTCTAATTACTTGTATATTAGTATATATGTTTATATTTATTTCAATGCAATTGCATG
*******************:*:****:******:*:***************:*:**

ARS at VII-112 has unique ID: 219

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VII-112 has unique ID: 219

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at VII-112 has unique ID: 219