Confirmed ARS at IV-749

ARS422.5

Other names: ARS451

Status: Confirmed ARS: Confirmed by ARS assay and identified by 6 genome-wide studies.

Genomic Location: Chr4:748384-748630
Within convergent intergenic space between YDR145W and YDR146C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -147.8 ΔG° (kcal/mol) at location 748624.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 32.3 min.
Yabuki et al. (2002) — Trep: 28.2 min.
Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-749 has unique ID: 123

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ARS at IV-749 has unique ID: 123

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:748384-748630


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr4:748195-749435

Szilard et al. (2010): Chr4:748635-748645


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:755444 (Trep: 32.3 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:751791 (Trep: 28.2 min.)

Alvino et al. (2007): Chr4:753710 (Peak first observed at 15.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr4:748384-748630 (Activity detected in: ddc1, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-749 has unique ID: 123

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTAATTAATATTTTGT                   
  Xu et al. (2006):      TGTTTTCATTTTGTTTTTATTATTAAATATTAAA  
  Xu et al. (2006):      AATTAATATTTTGTATCTAAAGTGTTCATACTAT  
ACS LOGO:   ACS_logo  

ARS at IV-749 has unique ID: 123

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeAATTTTTTAAAATTAATTGATTAATTAATATTTTGTATCTAAAGTGTTCATACTAT
S. kudriavzeviiGAAAAATTGAAGTTAATAAATA----AATGTTTTGTATCTTAATTGTTAATATTGC
S. mikataeAAATCCTTGAAATTAATTAGTA---------TTTGTATCTCAAATGTTCATGTTTT
S. paradoxusAAAAATTTCAAATTAATTAATTAATTAATATTTTGTACCTAAAATGTTCATACTGT
S. bayanusGAAATCTGTAAACTGATCAATT----AATATTTTGTGTCTCAA-TGTTCGT-----
*:*::**::*:**::*::::*****::****:****::*::

ARS at IV-749 has unique ID: 123

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-749 has unique ID: 123

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at IV-749 has unique ID: 123