ARS221

Status: Confirmed ARS: Confirmed by ARS assay and identified by 5 genome-wide studies.

Genomic Location: Chr2:631934-632246
Within convergent intergenic space between YBR203W and YBR204C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -145.5 ΔG° (kcal/mol) at location 632174.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 30.1 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at II-632 has unique ID: 51

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ARS at II-632 has unique ID: 51

Studies that cloned this origin

Xu et al. (2006): Chr2:631934-632246

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr2:631897-632209

Xu et al. (2006): Chr2:631365-633110

Szilard et al. (2010): Chr2:632075-632085

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr2:637508 (Trep: 30.1 min.) (Confidence: 1)

Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr2:631934-632246 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at II-632 has unique ID: 51

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Xu et al. (2006):

C

A

T

A

G

A

C

A

C

T

A

C

C

T

T

T

G

A

T

T

T

T

T

G

C

T

T

G

T

A

A

G

A

T

Xu et al. (2006):

T

T

A

T

T

T

A

T

T

T

A

T

A

T

T

T

A

T

T

T

T

T

C

G

T

T

T

C

C

T

A

T

T

T

ACS LOGO:

ARS at II-632 has unique ID: 51

Alignments from the UCSC genome browser

ARS at II-632 has unique ID: 51

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at II-632 has unique ID: 51

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

ARS at II-632 has unique ID: 51