Confirmed ARS at XV-437

ARS1513.5

Other names: ARS1501

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr15:436732-436966
Within divergent intergenic space between YOR058C and TK(UUU)O.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -129.6 ΔG° (kcal/mol) at location 436802.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 19.0 min.
Yabuki et al. (2002) — Trep: 20.6 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XV-437 has unique ID: 558

Loading - Please wait...

ARS at XV-437 has unique ID: 558

Studies that cloned this origin

Nieduszynski et al. (2006): Chr15:436732-436966


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Shor et al. (2009): Chr15:436500-437500 (orc2-1/wt peak ratio: 0.43)

Szilard et al. (2010): Chr15:436825-436835

Müller et al. (2010): Chr15:436400-437750 (orc1-bah-delta/wt peak ratio: 0.60)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr15:441255 (Trep: 19.0 min.) (Confidence: 9)

Yabuki et al. (2002): Chr15:436221 (Trep: 20.6 min.)

Alvino et al. (2007): Chr15:436250 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr15:437500 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr15:436732-436966 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XV-437 has unique ID: 558

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       ATATTTATTTTTATTC                   
  Eaton et al. (2010):      TTGATTAATATTTTGTTGAAAAGTAAAAGTTTT   
ACS LOGO:   ACS_logo  

ARS at XV-437 has unique ID: 558

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeTATTAGCTGGTTTTAAATCA
S. mikataeTATCAACTAAAGTCATACTA
S. paradoxusTAT--------------TTA
***:::::::*

ARS at XV-437 has unique ID: 558

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XV-437 has unique ID: 558

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at XV-437 has unique ID: 558