OriDB - ARS1322

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Origin

Status: Confirmed ARS: Confirmed by ARS assay and identified by 7 genome-wide studies.

Genomic Location: Chr13:554392-554750
Within convergent intergenic space between YMR144W and YMR145C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -137.4 ΔG° (kcal/mol) at location 554412.

Time of Origin Replication (Trep): This site was not identified as an origin by any curated timing study (Raghuraman et al. (2001); Yabuki et al. (2002); Alvino et al. (2007)), suggesting that this site is not chromosomally active for replication initiation.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XIII-555 has unique ID: 478

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ARS at XIII-555 has unique ID: 478

Studies that cloned this origin

Liachko et al. (2010): Chr13:554392-554750

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr13:553421-554330

Xu et al. (2006): Chr13:553565-556235

Shor et al. (2009): Chr13:554100-556400 (orc2-1/wt peak ratio: 0.53)

Szilard et al. (2010): Chr13:554435-554445

MÃ¼ller et al. (2010): Chr13:553925-555425 (orc1-bah-delta/wt peak ratio: 0.59)

Studies that measured the replication time of this origin

The replication time of origin has not be reported by any curated study.

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr13:555000 (Activity detected in: rad53)

CrabbÃ© et al. (2010): Chr13:554612-555112 (Activity detected in: rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)