ARS804

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr8:45589-46076
Within convergent intergenic space between YHL030W and YHL029C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -149.4 ΔG° (kcal/mol) at location 46029.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 36.4 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VIII-46 has unique ID: 266

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ARS at VIII-46 has unique ID: 266

Studies that cloned this origin

Müller & Nieduszynski (2012): Chr8:45589-46076

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr8:45688-45927

Xu et al. (2006): Chr8:45115-47110

Shor et al. (2009): Chr8:45100-46500 (orc2-1/wt peak ratio: 0.42)

Szilard et al. (2010): Chr8:45635-45645

Müller et al. (2010): Chr8:45425-46400 (orc1-bah-delta/wt peak ratio: 1.09)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr8:45788 (Trep: 36.4 min.) (Confidence: 4)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr8:46000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr8:45844-46344 (Activity detected in: ddc1, rad24, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VIII-46 has unique ID: 266

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Xu et al. (2006):

A

A

A

G

T

T

A

A

T

A

T

T

T

A

G

T

A

C

C

G

A

T

A

C

T

A

T

A

C

A

T

T

A

T

Eaton et al. (2010):

A

A

A

G

T

T

A

A

T

A

T

T

T

A

G

T

A

C

C

G

A

T

A

C

T

A

T

A

C

A

T

T

A

ACS LOGO:

ARS at VIII-46 has unique ID: 266

Alignments from the UCSC genome browser

ARS at VIII-46 has unique ID: 266

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VIII-46 has unique ID: 266

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Müller & Nieduszynski (2012): PubMed | Genome Res.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VIII-46 has unique ID: 266