ARS315

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 9 genome-wide studies.

Genomic Location: Chr3:224807-225053
Within tandem intergenic space between YCR060W and YCR061W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -144.6 ΔG° (kcal/mol) at location 224867.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 14.8 min.
Yabuki et al. (2002) — Trep: 24.2 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-225 has unique ID: 85

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ARS at III-225 has unique ID: 85

Studies that cloned this origin

Poloumienko et al. (2001): Chr3:224796-225360

Nieduszynski et al. (2006): Chr3:224807-225053

Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): Chr3:222368-226808

Tourrière et al. (2005): Chr3:222374-226803

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr3:223725-225575

Shor et al. (2009): Chr3:224300-225300 (orc2-1/wt peak ratio: 0.66)

Szilard et al. (2010): Chr3:224975-224985

Müller et al. (2010): Chr3:224300-225125 (orc1-bah-delta/wt peak ratio: 0.76)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:227557 (Trep: 14.8 min.) (Confidence: 9)

Yabuki et al. (2002): Chr3:222863 (Trep: 24.2 min.)

Alvino et al. (2007): Chr3:221330 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:224666 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr3:224807-225053 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at III-225 has unique ID: 85

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

T

T

T

T

T

T

A

T

G

T

T

T

T

T

C

T

Xu et al. (2006):

T

T

T

T

A

T

G

T

T

T

T

T

C

T

T

C

G

C

G

C

G

T

C

A

A

C

T

T

T

C

T

A

C

C

ACS LOGO:

ARS at III-225 has unique ID: 85

Alignments from the UCSC genome browser

ARS at III-225 has unique ID: 85

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at III-225 has unique ID: 85

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Poloumienko et al. (2001): PubMed | PubMed Central

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): PubMed | PubMed Central

Tourrière et al. (2005): PubMed | Mol. Cell

Studies that confirmed an essential ACS element

Crampton et al. (2008): PubMed | Mol. Cell

Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

ARS at III-225 has unique ID: 85