ARS1007.5

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 6 genome-wide studies.

Genomic Location: Chr10:161435-161860
Within tandem intergenic space between YJL133W and YJL132W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -153.2 ΔG° (kcal/mol) at location 161835.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 26.5 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-162 has unique ID: 334

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ARS at X-162 has unique ID: 334

Studies that cloned this origin

Xu et al. (2006): Chr10:161435-161860

Studies that analyzed this origin by 2D gel

Feng et al. (2006): Chr10:159458-162730

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr10:160725-162685

Szilard et al. (2010): Chr10:161535-161545

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:160753 (Trep: 26.5 min.) (Confidence: 9)

Alvino et al. (2007): Chr10:162500 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:161333 (Activity detected in: rad53)

Crabbé et al. (2010): Chr10:161435-161860 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at X-162 has unique ID: 334

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

A

C

T

T

C

T

A

T

T

G

T

A

A

C

A

A

A

A

T

T

G

C

A

A

G

C

T

G

C

T

G

G

A

G

ACS LOGO:

ARS at X-162 has unique ID: 334

Alignments from the UCSC genome browser

ARS at X-162 has unique ID: 334

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-162 has unique ID: 334

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Studies that analyzed this origin by 2D gel

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Studies that confirmed an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

ARS at X-162 has unique ID: 334