OriDB - ARS317

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 8 genome-wide studies.

Genomic Location: Chr3:292524-292826
Within tandem intergenic space between YCR095C and YCR096C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -137.7 ΔG° (kcal/mol) at location 292694.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 31.1 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-293 has unique ID: 87

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ARS at III-293 has unique ID: 87

Studies that cloned this origin

Poloumienko et al. (2001): Chr3:292379-292917

Nieduszynski et al. (2006): Chr3:292524-292826

Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): Chr3:290914-294308

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:293172-293531

Wyrick et al. (2001): Chr3:293043-293725

Shor et al. (2009): Chr3:291000-293900 (orc2-1/wt peak ratio: 1.01)

Szilard et al. (2010): Chr3:292465-292475

MÃ¼ller et al. (2010): Chr3:291575-293375 (orc1-bah-delta/wt peak ratio: 0.73)

Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr3:294863 (Trep: 31.1 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:294500 (Activity detected in: rad53)

CrabbÃ© et al. (2010): Chr3:292524-292826 (Activity detected in: ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at III-293 has unique ID: 87

Studies that confirmed an essential ACS element

Chang et al. (2008):

T

A

T

A

T

A

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

T

G

T

A

C

T

C

T

Eaton et al. (2010):

A

T

A

T

A

T

A

G

T

A

T

A

T

G

C

G

A

T

A

ACS LOGO:

ARS at III-293 has unique ID: 87

Alignments from the UCSC genome browser

Predicted ACS

S. cerevisiae

T

A

T

A

T

A

T

A

T

A

T

A

T

A

T

A

T

A

T

G

T

A

C

T

C

T

A

T

C

A

G

T

G

T

-

S. bayanus

C

A

T

A

T

G

T

A

T

A

C

T

G

T

A

T

G

T

A

T

C

T

C

T

A

C

A

T

A

C

-

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

ARS at III-293 has unique ID: 87

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at III-293 has unique ID: 87

ARS at III-293 has unique ID: 87

Confirmed ARS at III-293

ARS317

Other names: proARS317, proARS318

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Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Studies that measured the replication time of this origin

Studies that measured the activity of this origin in hydroxyurea (HU)

Studies that predicted the location of this origin

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Alignments from the UCSC genome browser

Genome-wide studies that identified this origin

Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element