Confirmed ARS at III-293

ARS317

Other names: proARS317, proARS318

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 8 genome-wide studies.

Genomic Location: Chr3:292524-292826
Within tandem intergenic space between YCR095C and YCR096C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -137.7 ΔG° (kcal/mol) at location 292694.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 31.1 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-293 has unique ID: 87

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ARS at III-293 has unique ID: 87

Studies that cloned this origin

Poloumienko et al. (2001): Chr3:292379-292917

Nieduszynski et al. (2006): Chr3:292524-292826


Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): Chr3:290914-294308


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:293172-293531

Wyrick et al. (2001): Chr3:293043-293725

Shor et al. (2009): Chr3:291000-293900 (orc2-1/wt peak ratio: 1.01)

Szilard et al. (2010): Chr3:292465-292475

Müller et al. (2010): Chr3:291575-293375 (orc1-bah-delta/wt peak ratio: 0.73)


Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr3:294863 (Trep: 31.1 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:294500 (Activity detected in: rad53)

Crabbé et al. (2010): Chr3:292524-292826 (Activity detected in: ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at III-293 has unique ID: 87

Studies that confirmed an essential ACS element

  Chang et al. (2008):         TTTTTATATTTA                     

Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTTGTTTACTTTTTCT                   
  Eaton et al. (2010):      ATTTTTTATATTTAGGTATTAAATTGCGATTTA   
ACS LOGO:   ACS_logo  

ARS at III-293 has unique ID: 87

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeTATATATATATATATATTTATTTGTTTACTTTTTCTATCAGTGT------------
S. bayanusCATAAATGTTTATACTGGTATTTGTTTATCTTTTCTAACATTAC------------
:***:**:*:****::::**********::*******:**:*::

ARS at III-293 has unique ID: 87

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at III-293 has unique ID: 87

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Poloumienko et al. (2001): PubMed | PubMed Central

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): PubMed | PubMed Central


Studies that confirmed an essential ACS element

Chang et al. (2008): PubMed | PubMed Central | Mol. Cell. Biol.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at III-293 has unique ID: 87