Confirmed ARS at XII-928

ARS1229

Other names: proARS1229

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr12:928051-928540
Within tandem intergenic space between YLR403W and YLR404W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -142.9 ΔG° (kcal/mol) at location 928371.

Time of Origin Replication (Trep): Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XII-928 has unique ID: 439

Loading - Please wait...

ARS at XII-928 has unique ID: 439

Studies that cloned this origin

Müller & Nieduszynski (2012): Chr12:928051-928540


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr12:927615-928739

Xu et al. (2006): Chr12:927045-929290

Shor et al. (2009): Chr12:927300-928900 (orc2-1/wt peak ratio: 0.35)

Szilard et al. (2010): Chr12:928315-928325

Müller et al. (2010): Chr12:927500-928925 (orc1-bah-delta/wt peak ratio: 0.71)


Studies that measured the replication time of this origin

Alvino et al. (2007): Chr12:931750 (Peak first observed at 15.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr12:929333 (Activity detected in: rad53)

Crabbé et al. (2010): Chr12:928095-928595 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XII-928 has unique ID: 439

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Xu et al. (2006):      ATATTATAGTTTTGTTAGTAATGTTGAACTTTTA  
  Eaton et al. (2010):      AATATTATAGTTTTGTTAGTAATGTTGAACTTT   
ACS LOGO:   ACS_logo  

ARS at XII-928 has unique ID: 439

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at XII-928 has unique ID: 439

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XII-928 has unique ID: 439

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Müller & Nieduszynski (2012): PubMed | Genome Res.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at XII-928 has unique ID: 439