OriDB - ARS1011

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 10 genome-wide studies.

Genomic Location: Chr10:336976-337225
Within convergent intergenic space between YJL053W and YJL052C-A.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -147.2 ΔG° (kcal/mol) at location 337106.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 31.4 min.
Yabuki et al. (2002) — Trep: 29.4 min.
Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-337 has unique ID: 342

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ARS at X-337 has unique ID: 342

Studies that cloned this origin

Nieduszynski et al. (2006): Chr10:336976-337225

Studies that analyzed this origin by 2D gel

Gibson et al. (2004): Chr10:335824-338216

Hu & Aparicio (2005): Chr10:335824-338216

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:336733-337967

Xu et al. (2006): Chr10:335845-339105

Shor et al. (2009): Chr10:336300-337800 (orc2-1/wt peak ratio: 0.98)

Szilard et al. (2010): Chr10:336995-337005

MÃ¼ller et al. (2010): Chr10:336350-337850 (orc1-bah-delta/wt peak ratio: 0.64)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:338258 (Trep: 31.4 min.) (Confidence: 8)

Yabuki et al. (2002): Chr10:335933 (Trep: 29.4 min.)

Alvino et al. (2007): Chr10:337400 (Peak first observed at 15.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:337000 (Activity detected in: rad53)

CrabbÃ© et al. (2010): Chr10:336976-337225 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at X-337 has unique ID: 342

Studies that confirmed an essential ACS element

Xu et al. (2006):

T

A

T

G

T

A

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

T

A

T

G

T

A

G

C

Eaton et al. (2010):

T

A

T

G

T

A

G

C

T

A

G

T

A

G

C

A

G

C

T

G

ACS LOGO:

ARS at X-337 has unique ID: 342

Alignments from the UCSC genome browser

Predicted ACS

S. cerevisiae

G

T

A

T

G

C

A

T

A

G

A

T

A

T

C

T

A

T

G

T

A

G

C

T

A

G

T

A

G

C

A

G

C

T

G

A

G

S. kudriavzevii

-

T

A

G

T

A

T

A

C

G

A

T

G

C

A

C

G

T

A

-

T

C

A

C

G

C

T

A

G

C

T

-

T

G

A

C

G

C

T

C

-

S. mikatae

G

T

A

T

A

G

T

C

A

C

A

C

T

C

-

C

T

G

T

A

C

A

-

C

A

T

G

-

S. paradoxus

G

T

A

T

G

T

A

T

-

G

T

A

C

A

T

A

C

T

C

T

A

T

G

T

A

G

C

A

T

A

T

A

G

T

A

G

C

T

A

G

A

G

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

ARS at X-337 has unique ID: 342

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-337 has unique ID: 342

ARS at X-337 has unique ID: 342

Confirmed ARS at X-337

ARS1011

Other names: proARS1011

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Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Studies that measured the replication time of this origin

Studies that measured the activity of this origin in hydroxyurea (HU)

Studies that predicted the location of this origin

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Alignments from the UCSC genome browser

Genome-wide studies that identified this origin

Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element