ARS908

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr9:80058-80557
Within convergent intergenic space between YIL144W and YIL143C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -153.5 ΔG° (kcal/mol) at location 80168.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 33.2 min.
Yabuki et al. (2002) — Trep: 25.8 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IX-80 has unique ID: 300

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ARS at IX-80 has unique ID: 300

Studies that cloned this origin

Müller & Nieduszynski (2012): Chr9:80058-80557

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr9:80149-80510

Xu et al. (2006): Chr9:79788-81342

Szilard et al. (2010): Chr9:80285-80295

Müller et al. (2010): Chr9:79700-80675 (orc1-bah-delta/wt peak ratio: 0.73)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr9:87252 (Trep: 33.2 min.) (Confidence: 2)

Yabuki et al. (2002): Chr9:77091 (Trep: 25.8 min.)

Alvino et al. (2007): Chr9:78250 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr9:79786-81344 (Activity detected in: ctf4, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at IX-80 has unique ID: 300

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Xu et al. (2006):

T

T

T

T

T

T

T

T

T

A

T

T

T

T

T

T

A

T

A

T

T

A

T

G

G

A

A

G

A

A

A

A

T

G

ACS LOGO:

ARS at IX-80 has unique ID: 300

Alignments from the UCSC genome browser

ARS at IX-80 has unique ID: 300

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IX-80 has unique ID: 300

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Müller & Nieduszynski (2012): PubMed | Genome Res.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

ARS at IX-80 has unique ID: 300