Confirmed ARS at IV-1159

ARS432

Other names: proARS432

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr4:1159250-1159499
Within tandem intergenic space between YDR342C and YDR343C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -148.6 ΔG° (kcal/mol) at location 1159330.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 12.6 min.
Yabuki et al. (2002) — Trep: 17.0 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-1159 has unique ID: 142

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ARS at IV-1159 has unique ID: 142

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:1159250-1159499


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr4:1158415-1159643

Xu et al. (2006): Chr4:1158100-1159700

Shor et al. (2009): Chr4:1159000-1159800 (orc2-1/wt peak ratio: 0.46)

Szilard et al. (2010): Chr4:1159385-1159395

Müller et al. (2010): Chr4:1159025-1159775 (orc1-bah-delta/wt peak ratio: 0.24)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:1164550 (Trep: 12.6 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:1162291 (Trep: 17.0 min.)

Alvino et al. (2007): Chr4:1162400 (Peak first observed at 10.0 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr4:1159250-1159499 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-1159 has unique ID: 142

Studies that confirmed an essential ACS element

  Nieduszynski et al. (2006):       ATTTTTTTTCTTTTCT                   

Studies that predicted an essential ACS element

None curated.

ACS LOGO:   ACS_logo  


ARS at IV-1159 has unique ID: 142

Alignments from the UCSC genome browser

Confirmed ACS
S. cerevisiae-------------TTTATTAATTTTTTTTCTTTTCTAAGCTTTGAAGAAAAGTTAA
S. kudriavzevii-------------TTTTTTAATTTTTTTTCCTTCCTCAGCTTTGGAGGGAGTTCAA
S. mikatae-------------TTTATTAATTTGTTTTCTTTTCCTAGTTTTGAGAAATATTTAA
S. paradoxus-------------TTTATTAATTTTCTTTCTTTTCTAAGCTTTGAAGAGAAGTTAA
S. bayanus-------------TTAACTAATTATTCTTTTATTTTACGCATTGCGAGAAGGTCAA
**:::*****::::**:::*::::*::***:***

ARS at IV-1159 has unique ID: 142

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-1159 has unique ID: 142

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that predicted an essential ACS element

None identified.


ARS at IV-1159 has unique ID: 142