OriDB - ARS316

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 9 genome-wide studies.

Genomic Location: Chr3:272844-273088
Within divergent intergenic space between YCR090C and YCR091W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -138.0 ΔG° (kcal/mol) at location 272844.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 33.4 min.
Yabuki et al. (2002) — Trep: 30.1 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-273 has unique ID: 86

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ARS at III-273 has unique ID: 86

Studies that cloned this origin

Poloumienko et al. (2001): Chr3:272667-273858

Nieduszynski et al. (2006): Chr3:272844-273088

Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): Chr3:270021-275960

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:271586-272356

Xu et al. (2006): Chr3:271685-273665

Szilard et al. (2010): Chr3:272995-273005

Müller et al. (2010): Chr3:273050-273575 (orc1-bah-delta/wt peak ratio: 0.45)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:276624 (Trep: 33.4 min.) (Confidence: 9)

Yabuki et al. (2002): Chr3:271863 (Trep: 30.1 min.)

Alvino et al. (2007): Chr3:277000 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:272000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr3:272844-273088 (Activity detected in: mrc1-AQ, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at III-273 has unique ID: 86

Studies that confirmed an essential ACS element

Chang et al. (2008):

T

C

A

T

A

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

T

C

A

T

A

G

T

Xu et al. (2006):

T

C

A

T

A

G

T

C

T

C

G

T

G

C

T

A

C

A

ACS LOGO:

ARS at III-273 has unique ID: 86

Alignments from the UCSC genome browser

Predicted ACS

S. cerevisiae

C

A

G

T

A

T

C

A

T

A

G

T

C

T

C

G

T

G

C

T

A

C

A

T

C

S. kudriavzevii

-

A

T

A

T

G

-

T

A

T

C

T

C

A

T

A

G

T

G

C

T

C

T

C

T

A

G

C

T

A

S. mikatae

-

C

T

C

T

A

G

A

T

A

G

T

C

T

A

C

G

T

C

A

C

T

C

T

S. paradoxus

C

A

T

A

T

C

-

T

A

T

C

A

T

A

G

T

C

G

T

C

G

T

A

C

T

A

C

T

G

S. bayanus

C

T

A

G

A

T

A

G

T

-

T

C

T

C

T

C

A

T

A

T

G

A

T

G

C

T

A

T

C

A

C

T

A

G

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

ARS at III-273 has unique ID: 86

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at III-273 has unique ID: 86

ARS at III-273 has unique ID: 86

Confirmed ARS at III-273

ARS316

Other names: proARS316

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Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Studies that measured the replication time of this origin

Studies that measured the activity of this origin in hydroxyurea (HU)

Studies that predicted the location of this origin

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Alignments from the UCSC genome browser

Genome-wide studies that identified this origin

Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element