Confirmed ARS at IV-236

ARS409.5

Status: Confirmed ARS: Confirmed by ARS assay and identified by 6 genome-wide studies.

Genomic Location: Chr4:235935-236184
Within convergent intergenic space between YDL127W and YDL126C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -152.9 ΔG° (kcal/mol) at location 236175.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 33.2 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-236 has unique ID: 103

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ARS at IV-236 has unique ID: 103

Studies that cloned this origin

Shor et al. (2009): Chr4:235935-236184


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr4:235365-236485

Shor et al. (2009): Chr4:236300-238400 (orc2-1/wt peak ratio: 0.81)

Szilard et al. (2010): Chr4:235975-235985


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:240812 (Trep: 33.2 min.) (Confidence: 9)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr4:235000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr4:235363-236487 (Activity detected in: ctf4, pol2-12, elg1, tof1, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-236 has unique ID: 103

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Xu et al. (2006):      CCCTTTTTATATTTATTTTCAAATTTGTCAAAAA  
ACS LOGO:   ACS_logo  

ARS at IV-236 has unique ID: 103

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at IV-236 has unique ID: 103

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-236 has unique ID: 103

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at IV-236 has unique ID: 103