Confirmed ARS at IV-46

ARS404

Other names: HO, proARS404

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr4:46181-46237
Within convergent intergenic space between YDL229W and YDL227C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -149.3 ΔG° (kcal/mol) at location 46231.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 36.5 min.
Yabuki et al. (2002) — Trep: 27.4 min.
Alvino et al. (2007) — Peak first observed at 17.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-46 has unique ID: 94

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ARS at IV-46 has unique ID: 94

Studies that cloned this origin

Xu et al. (2006): Chr4:46181-46237


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr4:45919-46272

Xu et al. (2006): Chr4:44390-48176

Shor et al. (2009): Chr4:45200-47700 (orc2-1/wt peak ratio: 0.58)

Szilard et al. (2010): Chr4:46285-46295

Müller et al. (2010): Chr4:45800-47300 (orc1-bah-delta/wt peak ratio: 0.63)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:43761 (Trep: 36.5 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:46791 (Trep: 27.4 min.)

Alvino et al. (2007): Chr4:47429 (Peak first observed at 17.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr4:47333 (Activity detected in: rad53)

Crabbé et al. (2010): Chr4:46181-46237 (Activity detected in: ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-46 has unique ID: 94

Studies that confirmed an essential ACS element

  Kearsey (1984):         ATTTAATATTTT                     

Studies that predicted an essential ACS element

  Xu et al. (2006):      AAATTTAATATTTTGGATGAAAAAAACCATTTTT  
  Eaton et al. (2010):      AAATTTAATATTTTGGATGAAAAAAACCATTTT   
ACS LOGO:   ACS_logo  

ARS at IV-46 has unique ID: 94

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at IV-46 has unique ID: 94

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-46 has unique ID: 94

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Kearsey (1984): PubMed


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at IV-46 has unique ID: 94