Confirmed ARS at XV-908

ARS1528

Other names: proARS1528

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr15:908288-908537
Within divergent intergenic space between YOR316C-A and YOR317W.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -159.9 ΔG° (kcal/mol) at location 908528.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 21.3 min.
Yabuki et al. (2002) — Trep: 21.7 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XV-908 has unique ID: 580

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ARS at XV-908 has unique ID: 580

Studies that cloned this origin

Nieduszynski et al. (2006): Chr15:908288-908537


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr15:907546-908440

Xu et al. (2006): Chr15:906225-909035

Shor et al. (2009): Chr15:907700-908900 (orc2-1/wt peak ratio: 0.95)

Szilard et al. (2010): Chr15:908375-908385

Müller et al. (2010): Chr15:907625-908900 (orc1-bah-delta/wt peak ratio: 0.64)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr15:907760 (Trep: 21.3 min.) (Confidence: 9)

Yabuki et al. (2002): Chr15:911721 (Trep: 21.7 min.)

Alvino et al. (2007): Chr15:908780 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr15:908000 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr15:908288-908537 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XV-908 has unique ID: 580

Studies that confirmed an essential ACS element

  Breier et al. (2004):      TTTGTTTATGTTTAGGT                   

Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTGTTTATGTTTAGGT                   
  Xu et al. (2006):      TGTTTATGTTTAGGTTAACGATAGACATATTTAT  
  Eaton et al. (2010):      TTTGTTTATGTTTAGGTTAACGATAGACATATT   
ACS LOGO:   ACS_logo  

ARS at XV-908 has unique ID: 580

Alignments from the UCSC genome browser

Predicted ACS
*

ARS at XV-908 has unique ID: 580

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XV-908 has unique ID: 580

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Breier et al. (2004): PubMed | PubMed Central | Genome Biol.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at XV-908 has unique ID: 580