Confirmed ARS at V-288

ARS514

Other names: proARS514

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr5:287504-287750
Within tandem intergenic space between YER065C and TS(UGA)E.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -146.2 ΔG° (kcal/mol) at location 287714.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 22.6 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at V-288 has unique ID: 181

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ARS at V-288 has unique ID: 181

Studies that cloned this origin

Nieduszynski et al. (2006): Chr5:287504-287750


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr5:286912-287743

Xu et al. (2006): Chr5:286005-288945

Shor et al. (2009): Chr5:286900-288700 (orc2-1/wt peak ratio: 1.06)

Szilard et al. (2010): Chr5:287615-287625

Müller et al. (2010): Chr5:286850-288650 (orc1-bah-delta/wt peak ratio: 0.88)


Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr5:287386 (Trep: 22.6 min.)

Alvino et al. (2007): Chr5:285120 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr5:287504-287750 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at V-288 has unique ID: 181

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTTATTTATGTTTTGT                   
  Xu et al. (2006):      TTTATTTATGTTTTGTTCCCAGCGGGCATCTTTC  
  Eaton et al. (2010):      TTTATTTATGTTTTGTTCCCAGCGGGCATCTTT   
ACS LOGO:   ACS_logo  

ARS at V-288 has unique ID: 181

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeGCTATATTGTACTCTTGAATTTTATTTATGTTTTGTTCCCAGCGGGCATCTTTCAA
S. kudriavzeviiACTC--TTGCACGCTCAGTTTTTTTTTATGTTTTGTTTGAGATGAAGAAAAATTAT
S. mikataeGCTCCACTGTATTCTTTATTCTTATTTATATTTTGTTGTAAAAAAAGATGAATAAA
S. paradoxusGCTATATTGCGCTCTT--ATTTTTTTTACATTTTGTTGCAAGAAAAAATCCCAGTG
:**::**:::**:*:******:*******:*:::

ARS at V-288 has unique ID: 181

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at V-288 has unique ID: 181

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at V-288 has unique ID: 181