ARS418

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr4:555224-555461
Within convergent intergenic space between YDR049W and YDR050C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -146.4 ΔG° (kcal/mol) at location 555344.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 18.0 min.
Yabuki et al. (2002) — Trep: 17.9 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-555 has unique ID: 114

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ARS at IV-555 has unique ID: 114

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:555224-555461

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr4:555192-555766

Xu et al. (2006): Chr4:554415-556395

Shor et al. (2009): Chr4:554700-556500 (orc2-1/wt peak ratio: 0.76)

Szilard et al. (2010): Chr4:555415-555425

Müller et al. (2010): Chr4:554750-555875 (orc1-bah-delta/wt peak ratio: 0.83)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:554392 (Trep: 18.0 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:555291 (Trep: 17.9 min.)

Alvino et al. (2007): Chr4:555560 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr4:553000 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr4:555224-555461 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at IV-555 has unique ID: 114

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

A

A

G

T

T

T

A

T

G

T

T

T

T

T

T

C

Xu et al. (2006):

T

G

A

A

A

G

T

T

T

A

T

G

T

T

T

T

T

T

C

A

C

T

G

G

A

G

G

C

G

T

C

A

T

T

Eaton et al. (2010):

A

A

A

G

T

T

T

A

T

G

T

T

T

T

T

T

C

A

C

T

G

G

A

G

G

C

G

T

C

A

T

T

T

ACS LOGO:

ARS at IV-555 has unique ID: 114

Alignments from the UCSC genome browser

ARS at IV-555 has unique ID: 114

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-555 has unique ID: 114

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at IV-555 has unique ID: 114