ARS609

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 8 genome-wide studies.

Genomic Location: Chr6:256263-256418
Within divergent intergenic space between YFR053C and YFR055W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -149.4 ΔG° (kcal/mol) at location 256263.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 31.8 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VI-256 has unique ID: 211

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ARS at VI-256 has unique ID: 211

Studies that cloned this origin

Shirahige et al. (1993): Chr6:256263-256418

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): Chr6:253670-259874

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr6:256305-257573

Xu et al. (2006): Chr6:255595-258310

Shor et al. (2009): Chr6:255800-258400 (orc2-1/wt peak ratio: 0.86)

Szilard et al. (2010): Chr6:256625-256635

Müller et al. (2010): Chr6:255650-257675 (orc1-bah-delta/wt peak ratio: 0.62)

Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr6:257363 (Trep: 31.8 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr6:257000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr6:256263-256418 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VI-256 has unique ID: 211

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

T

T

T

T

T

T

T

A

T

G

T

T

T

T

T

T

C

C

G

G

A

A

T

T

G

G

C

T

A

A

A

T

T

C

Eaton et al. (2010):

T

T

T

T

T

T

T

A

T

G

T

T

T

T

T

T

C

C

G

G

A

A

T

T

G

G

C

T

A

A

A

T

T

ACS LOGO:

ARS at VI-256 has unique ID: 211

Alignments from the UCSC genome browser

ARS at VI-256 has unique ID: 211

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VI-256 has unique ID: 211

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Shirahige et al. (1993): PubMed | PubMed Central

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): PubMed

Studies that confirmed an essential ACS element

Shirahige et al. (1993): PubMed | PubMed Central

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VI-256 has unique ID: 211