Status: Confirmed ARS: Confirmed by ARS assay and identified by 11 genome-wide studies.
Within convergent intergenic space between YCL066W and YCL064C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser
Origin Sequence Elements: ACS confirmed.
Helical Stability: Minimum -139.7 ΔG° (kcal/mol) at location 15000.
Time of Origin Replication (Trep): This site was not identified as an origin by any curated timing study (Raghuraman et al. (2001); Yabuki et al. (2002); Alvino et al. (2007)), suggesting that this site is not chromosomally active for replication initiation.
Origin activity in HU: Origin activity detected in HU — more details.
Chr3:9400-17000 (orc2-1/wt peak ratio: 0.79)
Chr3:12650-15950 (orc1-bah-delta/wt peak ratio: 0.31)
The replication time of origin has not be reported by any curated study.
Chr3:12666 (Activity detected in: rad53)
Chr3:14574-14849 (Activity detected in: ctf18 rad9, mec1-100, rad53, mec1)
This origin has not been predicted by any curated study.
|Vujcic et al. (1999):||T||A||T||T||T||A||T||A||T||T||T||T|
|Xu et al. (2006):||G||G||T||A||T||T||T||A||T||A||T||T||T||T||G||C||C||G||A||C||A||T||T||T||G||C||A||G||C||T||C||T||T||T|
These notes are manually curated. To submit notes for this replication origin site please contact us.
Friday, 18th August 2006 - Conrad Nieduszynski:
2D gel analysis indicates that ARS303 (like ARS301, ARS302 & ARS320) does NOT normally serve as chromosomal replication origin (see Dubey et al., 1991 in References tab). [Thanks to Carol Newlon for this information.]