ARS516

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr5:353504-353751
Within tandem intergenic space between YER096W and TE(UUC)E2.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -139.0 ΔG° (kcal/mol) at location 353744.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 24.3 min.
Yabuki et al. (2002) — Trep: 19.7 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at V-354 has unique ID: 184

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ARS at V-354 has unique ID: 184

Studies that cloned this origin

Nieduszynski et al. (2006): Chr5:353504-353751

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr5:353731-354132

Xu et al. (2006): Chr5:351715-355345

Shor et al. (2009): Chr5:352600-354300 (orc2-1/wt peak ratio: 0.88)

Szilard et al. (2010): Chr5:353735-353745

Müller et al. (2010): Chr5:352550-354650 (orc1-bah-delta/wt peak ratio: 0.57)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr5:350388 (Trep: 24.3 min.) (Confidence: 9)

Yabuki et al. (2002): Chr5:353386 (Trep: 19.7 min.)

Alvino et al. (2007): Chr5:353880 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr5:353500 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr5:353504-353751 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at V-354 has unique ID: 184

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

A

A

T

A

T

T

T

A

C

T

T

T

T

T

G

G

Xu et al. (2006):

A

A

T

A

T

T

T

A

C

T

T

T

T

T

G

G

T

G

C

G

G

A

A

A

T

C

T

G

T

A

A

T

A

T

Eaton et al. (2010):

A

A

T

A

T

T

T

A

C

T

T

T

T

T

G

G

T

G

C

G

G

A

A

A

T

C

T

G

T

A

A

T

A

ACS LOGO:

ARS at V-354 has unique ID: 184

Alignments from the UCSC genome browser

ARS at V-354 has unique ID: 184

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at V-354 has unique ID: 184

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at V-354 has unique ID: 184