OriDB - ARS718

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr7:421093-421342
Within divergent intergenic space between YGL040C and TL(CAA)G2.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -145.6 ΔG° (kcal/mol) at location 421283.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 30.8 min.
Yabuki et al. (2002) — Trep: 23.2 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VII-421 has unique ID: 234

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ARS at VII-421 has unique ID: 234

Studies that cloned this origin

Nieduszynski et al. (2006): Chr7:421093-421342

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr7:420552-421820

Xu et al. (2006): Chr7:420235-423540

Shor et al. (2009): Chr7:420700-422300 (orc2-1/wt peak ratio: 0.44)

Szilard et al. (2010): Chr7:421315-421325

Müller et al. (2010): Chr7:420575-422375 (orc1-bah-delta/wt peak ratio: 0.60)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr7:418930 (Trep: 30.8 min.) (Confidence: 9)

Yabuki et al. (2002): Chr7:418647 (Trep: 23.2 min.)

Alvino et al. (2007): Chr7:421500 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr7:420000 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr7:421093-421342 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VII-421 has unique ID: 234

Studies that confirmed an essential ACS element

Nieduszynski et al. (2006):

T

A

T

A

T

G

T

A

G

T

Studies that predicted an essential ACS element

Xu et al. (2006):

G

A

T

A

T

A

T

G

T

A

G

T

G

A

T

A

G

C

T

A

C

T

Eaton et al. (2010):

T

A

T

A

T

G

T

A

G

T

G

A

T

A

G

C

T

A

C

T

ACS LOGO:

ARS at VII-421 has unique ID: 234

Alignments from the UCSC genome browser

Confirmed ACS

S. cerevisiae

C

A

C

T

C

T

C

G

A

C

A

G

A

T

A

T

A

T

G

T

A

G

T

G

A

T

A

G

C

T

A

C

T

A

S. kudriavzevii

-

A

C

G

A

T

A

T

G

T

A

G

T

G

T

C

T

C

A

C

A

T

C

T

C

G

S. paradoxus

T

C

A

T

G

T

G

A

G

A

C

A

C

A

T

A

T

A

T

A

T

A

G

T

C

T

C

A

C

A

G

C

T

A

T

C

A

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

ARS at VII-421 has unique ID: 234

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VII-421 has unique ID: 234

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VII-421 has unique ID: 234

Confirmed ARS at VII-421

ARS718

Other names: proARS718

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Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Studies that measured the replication time of this origin

Studies that measured the activity of this origin in hydroxyurea (HU)

Studies that predicted the location of this origin

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Alignments from the UCSC genome browser

Genome-wide studies that identified this origin

Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element