ARS209

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr2:254890-255136
Within tandem intergenic space between YBR008C and YBR009C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -147.5 ΔG° (kcal/mol) at location 255070.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 25.1 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at II-255 has unique ID: 34

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ARS at II-255 has unique ID: 34

Studies that cloned this origin

Bouton & Smith (1986): Chr2:254744-255118

Nieduszynski et al. (2006): Chr2:254890-255136

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr2:254166-255327

Xu et al. (2006): Chr2:254755-256565

Shor et al. (2009): Chr2:254700-255800 (orc2-1/wt peak ratio: 0.80)

Szilard et al. (2010): Chr2:255085-255095

Müller et al. (2010): Chr2:254525-255875 (orc1-bah-delta/wt peak ratio: 0.57)

Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr2:258978 (Trep: 25.1 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr2:252666 (Activity detected in: rad53)

Crabbé et al. (2010): Chr2:254890-255136 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at II-255 has unique ID: 34

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

T

T

T

A

A

T

G

A

T

G

G

A

A

T

A

A

T

T

T

G

G

G

A

A

T

T

T

A

C

T

C

T

G

T

Xu et al. (2006):

T

C

T

T

T

A

T

T

T

A

C

T

T

T

C

T

A

A

A

A

T

C

C

A

A

A

T

A

C

A

A

A

A

C

Eaton et al. (2010):

T

T

T

A

T

T

T

A

T

T

T

T

T

A

T

G

T

T

T

T

G

T

A

T

T

T

G

G

A

T

T

T

T

ACS LOGO:

ARS at II-255 has unique ID: 34

Alignments from the UCSC genome browser

ARS at II-255 has unique ID: 34

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at II-255 has unique ID: 34

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Bouton & Smith (1986): PubMed | PubMed Central

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

Bouton & Smith (1986): PubMed | PubMed Central

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at II-255 has unique ID: 34