OriDB - ARS1525

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Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr15:854735-855228
Within tandem intergenic space between TA(UGC)O and YOR290C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -160.1 ΔG° (kcal/mol) at location 855145.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 17.7 min.
Yabuki et al. (2002) — Trep: 24.3 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XV-855 has unique ID: 577

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ARS at XV-855 has unique ID: 577

Studies that cloned this origin

MÃ¼ller & Nieduszynski (2012): Chr15:854735-855228

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr15:854609-855142

Xu et al. (2006): Chr15:854175-856025

Shor et al. (2009): Chr15:854600-855200 (orc2-1/wt peak ratio: 0.24)

Szilard et al. (2010): Chr15:854935-854945

MÃ¼ller et al. (2010): Chr15:854525-855650 (orc1-bah-delta/wt peak ratio: 0.42)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr15:854760 (Trep: 17.7 min.) (Confidence: 8)

Yabuki et al. (2002): Chr15:851721 (Trep: 24.3 min.)

Alvino et al. (2007): Chr15:854140 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr15:857000 (Activity detected in: rad53)

CrabbÃ© et al. (2010): Chr15:854298-854798 (Activity detected in: wild-type, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)