Confirmed ARS at IV-1110

ARS431.5

Other names: ARS452

Status: Confirmed ARS: Confirmed by ARS assay and identified by 7 genome-wide studies.

Genomic Location: Chr4:1109955-1110196
Within tandem intergenic space between YDR321W and YDR322W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -143.4 ΔG° (kcal/mol) at location 1109965.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 35.9 min.
Yabuki et al. (2002) — Trep: 28.4 min.
Alvino et al. (2007) — Peak first observed at 17.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-1110 has unique ID: 140

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ARS at IV-1110 has unique ID: 140

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:1109955-1110196


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr4:1109200-1110450

Szilard et al. (2010): Chr4:1110045-1110055


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:1109040 (Trep: 35.9 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:1111791 (Trep: 28.4 min.)

Alvino et al. (2007): Chr4:1111300 (Peak first observed at 17.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr4:1110000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr4:1109955-1110196 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-1110 has unique ID: 140

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTTTTTTATATTTTTA                   
  Xu et al. (2006):      AAAATTATTAATATTATTTTTTTTATATTTTTAT  
ACS LOGO:   ACS_logo  

ARS at IV-1110 has unique ID: 140

Alignments from the UCSC genome browser

Predicted ACS
*

ARS at IV-1110 has unique ID: 140

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-1110 has unique ID: 140

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at IV-1110 has unique ID: 140