Confirmed ARS at XII-645

ARS1219.7

Status: Confirmed ARS: Confirmed by ARS assay and identified by 4 genome-wide studies.

Genomic Location: Chr12:644937-645426
Within divergent intergenic space between YLR254C and YLR256W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -153.0 ΔG° (kcal/mol) at location 644957.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 29.2 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XII-645 has unique ID: 726

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ARS at XII-645 has unique ID: 726

Studies that cloned this origin

Müller & Nieduszynski (2012): Chr12:644937-645426


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr12:644495-645765

Szilard et al. (2010): Chr12:644945-644955


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr12:639533 (Trep: 29.2 min.) (Confidence: 3)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr12:644493-645767 (Activity detected in: ctf4, pol2-12, elg1, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XII-645 has unique ID: 726

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

None curated.

ACS LOGO:   ACS_logo  


ARS at XII-645 has unique ID: 726

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at XII-645 has unique ID: 726

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XII-645 has unique ID: 726

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Müller & Nieduszynski (2012): PubMed | Genome Res.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

None identified.


ARS at XII-645 has unique ID: 726