Confirmed ARS at IV-1487

ARS446

Other names: proARS446

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr4:1486905-1487149
Within tandem intergenic space between YDR523C and YDR524C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -145.0 ΔG° (kcal/mol) at location 1486905.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 35.9 min.
Yabuki et al. (2002) — Trep: 28.6 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-1487 has unique ID: 162

Loading - Please wait...

ARS at IV-1487 has unique ID: 162

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:1486905-1487149


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr4:1487086-1487590

Xu et al. (2006): Chr4:1485700-1488000

Shor et al. (2009): Chr4:1486700-1487400 (orc2-1/wt peak ratio: 0.29)

Szilard et al. (2010): Chr4:1486985-1486995

Müller et al. (2010): Chr4:1486775-1487450 (orc1-bah-delta/wt peak ratio: 0.21)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:1489130 (Trep: 35.9 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:1489791 (Trep: 28.6 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr4:1487000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr4:1486905-1487149 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-1487 has unique ID: 162

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       CTTTTTAAATTTAGTT                   
  Xu et al. (2006):      TTGCTTTTTAAATTTAGTTTTTTTACTAGCTAAT  
ACS LOGO:   ACS_logo  

ARS at IV-1487 has unique ID: 162

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeAGAAAGCAAACCAGCATTTGCTTTTTAAATTTAGTTTTTTTACTAGCTAATTCATT
S. kudriavzeviiAAAAAGCAAACCAGCGTTTGCGTTATAATTCTAGTCTTTT-ACTAGCTAACCAATT
S. paradoxusAGA--GCAAATCAGCATTTGCTTTTTAATTTTAGTTTTTTTACTGGCTAATTCATT
S. bayanusAAAGAGCAATCCGCCATTTGCGTTATAACTTTAGTTTATC-ATTAGCTAGATCATT
**:****::*::*:***********:****:*:*:*:*:****:::***

ARS at IV-1487 has unique ID: 162

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-1487 has unique ID: 162

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at IV-1487 has unique ID: 162