ARS1004

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr10:23661-24158
Within convergent intergenic space between YJL217W and YJL216C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -143.0 ΔG° (kcal/mol) at location 23751.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 38.7 min.
Alvino et al. (2007) — Peak first observed at 15.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-24 has unique ID: 329

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ARS at X-24 has unique ID: 329

Studies that cloned this origin

Wyrick et al. (2001): Chr10:23661-24158

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:23729-24341

Xu et al. (2006): Chr10:23258-25477

Shor et al. (2009): Chr10:23400-24300 (orc2-1/wt peak ratio: 0.11)

Szilard et al. (2010): Chr10:23955-23965

Müller et al. (2010): Chr10:23300-24425 (orc1-bah-delta/wt peak ratio: 0.48)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr10:27250 (Trep: 38.7 min.) (Confidence: 6)

Alvino et al. (2007): Chr10:24600 (Peak first observed at 15.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr10:24000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr10:23661-24158 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at X-24 has unique ID: 329

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

A

A

A

T

T

T

T

T

A

G

T

T

T

T

G

T

T

A

T

A

A

T

A

A

A

C

G

A

C

T

T

T

T

A

Xu et al. (2006):

C

T

T

T

T

T

A

A

A

A

T

T

T

T

G

T

T

T

A

T

A

C

T

C

A

A

T

T

T

C

G

T

C

A

Eaton et al. (2010):

A

A

A

T

T

T

T

T

A

G

T

T

T

T

G

T

T

A

T

A

A

T

A

A

A

C

G

A

C

T

T

T

T

ACS LOGO:

ARS at X-24 has unique ID: 329

Alignments from the UCSC genome browser

ARS at X-24 has unique ID: 329

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-24 has unique ID: 329

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Wyrick et al. (2001): PubMed | Science

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at X-24 has unique ID: 329