ARS605

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 9 genome-wide studies.

Genomic Location: Chr6:135979-136080
Within gene: YFL003C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -141.4 ΔG° (kcal/mol) at location 135979.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 25.4 min.
Yabuki et al. (2002) — Trep: 20.4 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VI-136 has unique ID: 206

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ARS at VI-136 has unique ID: 206

Studies that cloned this origin

Shirahige et al. (1993): Chr6:135979-136080

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): Chr6:133462-137882

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr6:135639-136459

Xu et al. (2006): Chr6:134675-137585

Shor et al. (2009): Chr6:135400-137600 (orc2-1/wt peak ratio: 0.46)

Szilard et al. (2010): Chr6:135935-135945

Müller et al. (2010): Chr6:135200-137525 (orc1-bah-delta/wt peak ratio: 0.41)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr6:138951 (Trep: 25.4 min.) (Confidence: 6)

Yabuki et al. (2002): Chr6:136363 (Trep: 20.4 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr6:135750 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr6:135979-136080 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VI-136 has unique ID: 206

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

A

T

T

A

A

T

T

A

C

G

T

T

T

T

G

T

T

G

C

T

A

A

A

G

G

A

A

A

C

T

T

T

A

C

Eaton et al. (2010):

A

T

T

A

A

T

T

A

C

G

T

T

T

T

G

T

T

G

C

T

A

A

A

G

G

A

A

A

C

T

T

T

A

ACS LOGO:

ARS at VI-136 has unique ID: 206

Alignments from the UCSC genome browser

ARS at VI-136 has unique ID: 206

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VI-136 has unique ID: 206

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Shirahige et al. (1993): PubMed | PubMed Central

Studies that analyzed this origin by 2D gel

Yamashita et al. (1997): PubMed

Studies that confirmed an essential ACS element

Shirahige et al. (1993): PubMed | PubMed Central

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VI-136 has unique ID: 206