Confirmed ARS at II-94

ARS203

Other names: proARS203

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr2:93410-93811
Within convergent intergenic space between YBL068W and YBL067C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -147.1 ΔG° (kcal/mol) at location 93700.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 31.5 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at II-94 has unique ID: 24

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ARS at II-94 has unique ID: 24

Studies that cloned this origin

Xu et al. (2006): Chr2:93410-93811


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr2:93389-93811

Xu et al. (2006): Chr2:92770-94474

Shor et al. (2009): Chr2:92900-94100 (orc2-1/wt peak ratio: 0.45)

Szilard et al. (2010): Chr2:93545-93555

Müller et al. (2010): Chr2:93050-94025 (orc1-bah-delta/wt peak ratio: 0.87)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr2:93788 (Trep: 31.5 min.) (Confidence: 7)

Alvino et al. (2007): Chr2:96500 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr2:94000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr2:93410-93811 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at II-94 has unique ID: 24

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Eaton et al. (2010):      AGATATTATATTTATGTAAGAATTAAATTAATA   
ACS LOGO:   ACS_logo  

ARS at II-94 has unique ID: 24

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at II-94 has unique ID: 24

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at II-94 has unique ID: 24

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at II-94 has unique ID: 24