Confirmed ARS at III-194

ARS313

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 7 genome-wide studies.

Genomic Location: Chr3:194256-194505
Within convergent intergenic space between YCR036W and YCR037C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -151.4 ΔG° (kcal/mol) at location 194396.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 16.9 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-194 has unique ID: 80

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ARS at III-194 has unique ID: 80

Studies that cloned this origin

Poloumienko et al. (2001): Chr3:194252-195043

Nieduszynski et al. (2006): Chr3:194256-194505


Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): Chr3:192312-195908


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr3:193670-201315

Shor et al. (2009): Chr3:193800-195900 (orc2-1/wt peak ratio: 0.50)

Szilard et al. (2010): Chr3:194385-194395

Müller et al. (2010): Chr3:193775-195125 (orc1-bah-delta/wt peak ratio: 0.82)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:200020 (Trep: 16.9 min.) (Confidence: 9)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:195000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr3:194256-194505 (Activity detected in: ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at III-194 has unique ID: 80

Studies that confirmed an essential ACS element

  Chang et al. (2008):         TTTTACTTTTAG                     

Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTTTTTACTTTTAGTT                   
  Xu et al. (2006):      TTTTACTTTTAGTTTGTTAAATTTTAGTTTTCGT  
  Eaton et al. (2010):      ATTTTTTACTTTTAGTTTGTTAAATTTTAGTTT   
ACS LOGO:   ACS_logo  

ARS at III-194 has unique ID: 80

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeATGCATAAAATCTACTGCAATTTTTTACTTTTAGTTTGTTAAATTTTAGTTTTCGT
S. kudriavzeviiTTGTATGAAGTATGTGTTAACTTTTTATTTTTAGTTTATAAAATGTCATTTTTCGT
S. mikataeTTTAATGAAATAGGTGACAA-CTTTTATTACTGATTT-TTATATGTTATGTTTCGT
S. paradoxusGTGTGT-AAATCTGTGATAATTTTTTATTTTTAGTTTATAAAATGTTGATTTTTGT
S. bayanusTGTTATGTAATTTGTTTTATTTTTTTATTTTCAGTTT------TTTTATTTTTTCT
::::*::*:*::::*::*****:*:::::***::::**::::***:*

ARS at III-194 has unique ID: 80

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at III-194 has unique ID: 80

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Poloumienko et al. (2001): PubMed | PubMed Central

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): PubMed | PubMed Central


Studies that confirmed an essential ACS element

Chang et al. (2008): PubMed | PubMed Central | Mol. Cell. Biol.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at III-194 has unique ID: 80