OriDB - ARS308

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Status: Confirmed ARS: Confirmed by ARS assay and identified by 6 genome-wide studies.

Genomic Location: Chr3:114314-114933
Within convergent intergenic space between YCL001W-B and YCR002C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -151.3 ΔG° (kcal/mol) at location 114914.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 19.8 min.
Yabuki et al. (2002) — Trep: 19.3 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-115 has unique ID: 74

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ARS at III-115 has unique ID: 74

Studies that cloned this origin

Newlon et al. (1991): Chr3:114314-114933

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr3:114695-115575

Szilard et al. (2010): Chr3:114795-114805

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:115906 (Trep: 19.8 min.) (Confidence: 9)

Yabuki et al. (2002): Chr3:109863 (Trep: 19.3 min.)

Alvino et al. (2007): Chr3:113440 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

CrabbÃ© et al. (2010): Chr3:114314-114933 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)