Confirmed ARS at VII-353

ARS716

Other names: proARS716

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 10 genome-wide studies.

Genomic Location: Chr7:352695-352917
Within divergent intergenic space between YGL084C and YGL083W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -143.5 ΔG° (kcal/mol) at location 352835.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 33.9 min.
Yabuki et al. (2002) — Trep: 28.2 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VII-353 has unique ID: 232

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ARS at VII-353 has unique ID: 232

Studies that cloned this origin

Nieduszynski et al. (2006): Chr7:352695-352917


Studies that analyzed this origin by 2D gel

Aparicio et al. (2004): Chr7:350378-355258


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr7:352296-353056

Xu et al. (2006): Chr7:352245-353635

Shor et al. (2009): Chr7:352400-353300 (orc2-1/wt peak ratio: 0.17)

Szilard et al. (2010): Chr7:352895-352905

Müller et al. (2010): Chr7:352475-353225 (orc1-bah-delta/wt peak ratio: 1.26)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr7:353902 (Trep: 33.9 min.) (Confidence: 9)

Yabuki et al. (2002): Chr7:350647 (Trep: 28.2 min.)

Alvino et al. (2007): Chr7:352860 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr7:351500 (Activity detected in: rad53)

Crabbé et al. (2010): Chr7:352695-352917 (Activity detected in: tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at VII-353 has unique ID: 232

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTAATTACGTTTAGTT                   
  Xu et al. (2006):      AGTTTAATTACGTTTAGTTTTCCTACTGAAACTT  
  Eaton et al. (2010):      TTTAATTACGTTTAGTTTTCCTACTGAAACTTT   
ACS LOGO:   ACS_logo  

ARS at VII-353 has unique ID: 232

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeGGTTCGAACCGAAGAATAGTTTAATTACGTTTAGTTTTCCTACTGAAACTTTTTTT
S. kudriavzeviiGGTTCGAACCGGATATAA----AATTACGTTTAGTTCTTCTTTTGAGCGCTTTTAG
S. mikataeGGTTCGAACCGAAAAAAAATTTAATCATGTTTAGTTTTTTTAAAAAAAACCTTCTA
S. paradoxusGGATCGAACCGAAGAACAGTTTAATTACGTTTAGTTTTCCTTCTGCATTTTTTTTC
S. bayanusGGTTCGAACCGAAAAAGAATAAAATCACGTTTAGTCTTCTTTCAAGGCATTTTTAC
**:********:**::*:****:*******::**::**::

ARS at VII-353 has unique ID: 232

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VII-353 has unique ID: 232

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

Aparicio et al. (2004): PubMed | PubMed Central | Mol. Cell. Biol.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at VII-353 has unique ID: 232